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微信小章| 产品名称： | SK-MEL-1 |
|---|---|
| 商品货号： | TS210911 |
| Organism： | Homo sapiens, human |
| Tissue： | malignant melanoma; skin; derived from metastatic site: lymphatic system |
| Cell Type： | Melanoma |
| Product Format： | frozen |
| Morphology： | spherical |
| Culture Properties： | clusters in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 29 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma. |
| Clinical Data： | 29 years
Caucasian
male |
| Antigen Expression： | Antigen expression: Blood Type A; Rh+ |
| Cellular Products： | melanin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters |
| Comments： | Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 105xa0cells/mL and maintain at 2 x 105xa0cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 12 THO1: 6 TPOX: 11 vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. PubMed: 4879578 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 |