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微信小章| 产品名称： | SK-LMS-1 |
|---|---|
| 商品货号： | TS210912 |
| Organism： | Homo sapiens, human |
| Tissue： | vulva |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leiomyosarcoma |
| Age： | 43 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The cell line is aneuploid human female, with chromosome counts in the triploid to hypertriploid range. Neither of the X chromosomes are normal, but their q arms are detected in a translocation with chromosome N4. Normal chromosomes N1, N4, N13, and N17 are clearly under-represented, while chromosomes N6, N18, and N20 are consistently over-represented with respect to the copy numbers of other normal chromosomes. Eight marker chromosomes are identified: 5p+, t(1pter--->cen::9q12--->9q33::3p21--->3pter), t(1p;?), der(2)t(1;2)(q21;q24), 12p+, der(6)t(6;?)(q21;?), der(4)t(X;4)(q13;q34), del(16)(p13). The cytogenetic and isozyme phenotype descriptors are in accord with those given by J. Fogh. |
| Clinical Data： | 43 years Caucasian female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms leiomyosarcoma |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12 D16S539: 8,11 D5S818: 11,13 D7S820: 8,9 THO1: 6,7 TPOX: 8,9 vWA: 18,20 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1971 |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765 Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031 |