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微信小章| 产品名称： | SK-MEL-24 |
|---|---|
| 商品货号： | TS210917 |
| Organism： | Homo sapiens, human |
| Tissue： | skin; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Morphology： | stellate |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Malignant melanoma |
| Age： | 67 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Derivation： | This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates. |
| Clinical Data： | 67 years Caucasian male |
| Antigen Expression： | Blood Type O; Rh+; HLA A1, A2, B12, B14, Cw5 |
| Tumorigenic： | Yes |
| Effects： | Yes, forms malignant melanoma |
| Complete Growth Medium： | The base medium for this cell line is Eagles Minimum Essential Medium (EMEM; ATCC 30-2003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (FBS; ATCC 30-2020) 15%. |
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 2 |
| Name of Depositor： | T Takahashi |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |