微信小陈
微信小章| 产品名称： | SHM-D33 |
|---|---|
| 商品货号： | TS210922 |
| Organism： | Homo sapiens; Mus musculus, human; mouse |
| Tissue： | heteromyeloma |
| Cell Type： | B lymphoblast; somatic cell hybridoma |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This line was produced by fusing the human myeloma cell line FU-266, clone E-1 (HAT sensitive, 8-azaguanine resistant and resistant to G-418 - an antibiotic similar to gentamicin) with the murine myeloma P3X63Ag8.653 (see ATCC CRL-1580). |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | HybriCare Medium (ATCC® No. 46X) supplemented with 20% high quality FBS (ATCC® 30-2020) and 0.2 mg/mL G418/Geneticin (Gibco 10131). |
| Subculturing： | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4xa0x 104 viable cells/mL.xa0Maintain cultures at a cell concentraton between 3xa0x 104 and 8xa0x 105 cells/mL.
Medium Renewal: Every 2 to 3 days
Note: The cells are grown in 200 to 400 mcg/mL of G-418 to maintain selection for the human chromosomes. |
| Cryopreservation： | Complete growth medium supplemented with 7.5% (v/v) DMSO |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 36 hours |
| Name of Depositor： | HS Kaplan |
| Deposited As： | Homo sapiens; Mus musculus |
| References： | Teng NN, et al. Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proc. Natl. Acad. Sci. USA 80: 7308-7312, 1983. PubMed: 6316357 Kearney JF, et al. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458 Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745 Yamagishi S, et al. Advanced glycation end products-driven angiogenesis in vitro. Induction of the growth and tube formation of human microvascular endothelial cells through autocrine vascular endothelial growth factor. J. Biol. Chem. 272: 8723-8730, 1997. PubMed: 9079706 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |