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| 产品名称： | SJCRH30 RC13, RMS 13, SJRH30 |
|---|---|
| 商品货号： | TS210925 |
| Organism： | Homo sapiens, human |
| Tissue： | muscle; derived from metastatic site: bone marrow |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | rhabdomyosarcoma |
| Age： | 17 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Derivation： | The line was established from cells from the bone marrow of a child with rhabdomyosarcoma.
Derived from metastatic site, bone marrow |
| Clinical Data： | 17 years
Caucasian
male
|
| Oncogene： | gli + (amplified 30 fold), N-myc + (not amplified) |
| Genes Expressed： | gli + (amplified 30 fold), N-myc + (not amplified) |
| Comments： | The cells show ultrastructural elements of primitive skeletal muscle differentiation. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Twice per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | D5S818: 12, 13 D13S317: 11 D7S820: 10 D16S539: 12 THO1: 9, 9.3 TPOX: 8, 11 vWA: 17, 18 CSF1PO: 10, 11 Amelogenin: XY |
| Name of Depositor： | DN Shapiro |
| Deposited As： | Homo sapiens |
| References： | Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537 Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305 Douglass EC, et al. A specific chromosomal abnormality in rhabdomyosarcoma published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179 |