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| 产品名称： | SJK-287-38 |
|---|---|
| 商品货号： | TS210926 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Antigen Expression： | H-2db |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human DNA polymerase alpha,H-2db |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human DNA polymerase alpha |
| Comments： | Mice were immunized with fraction VIII human DNA polymerase alpha. Spleen cells were fused with NS-1 myeloma cells. The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC TIB-179. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | D Korn |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672 |