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微信小章| 产品名称： | SH-34 |
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| 商品货号： | TS210935 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This monoclonal antibody can be used for the study of murine and rat hematopoietic and lymphopoietic cell lineages. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with the glycolipid asialo GM1 adsorbed to naked Salmonella. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against asialo-GM1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against asialo-GM1 |
| Comments： | The antibody is specific for asialo-GM1. This antibody recognizes and kills by complement lysis cells known to express asialo GM1.xa0
Most natural killer (NK) cells and cytotoxic T cells (CTL), as well as a proportion of other myeloid and lymphoid cells express asialo GM1.xa0 The SH-34 antibody specifically lyses asialo GM1-expressing macrophages in the presence of complement and removes NK cells in vitro from spleen cell populations. Ref
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| Complete Growth Medium： | Modified Dulbeccos medium, 95%; fetal bovine serum, 5%
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgM |
| Name of Depositor： | TJ Higgins |
| References： | Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562 Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206 Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802 |
Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206