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| 产品名称： | SC-1 |
|---|---|
| 商品货号： | TS210938 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | Initial cultures from a thawed ampule may take 10 to 12 days to become confluent. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 3 minutes, remove trypsin and let the culture sit at 37°C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Name of Depositor： | JW Hartley |
| Deposited As： | Mus musculus |
| References： | Hartley JW, Rowe WP. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65: 128-134, 1975. PubMed: 167514 |