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| 产品名称： | SCC-25 |
|---|---|
| 商品货号： | TS210942 |
| Organism： | Homo sapiens, human |
| Tissue： | tongue |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 70 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | male
70 years |
| Genes Expressed： | epidermal keratins; low levels of involucrin |
| Cellular Products： | epidermal keratins; low levels of involucrin |
| Tumorigenic： | Yes |
| Effects： | Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | SCC-25 cells are capable of only limited proliferation in semi-solid medium. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemented with 400 ng/ml hydrocortisone and 10% fetal bovine serum
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Note: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer. |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8 TPOX: 8,12 vWA: 17,19 |
| Name of Depositor： | JG Rheinwald |
| Deposited As： | Homo sapiens |
| References： | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 |