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| 产品名称： | SCC-9 |
|---|---|
| 商品货号： | TS210945 |
| Organism： | Homo sapiens, human |
| Tissue： | tongue |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 25 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Clinical Data： | male
25 years |
| Genes Expressed： | epidermal keratins; low levels of involucrin |
| Cellular Products： | epidermal keratins; low levels of involucrin |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107xa0cells. |
| Comments： | Growth of SCC-9 is enhanced by using a feeder layer of Mitomycin C-treated STO cells (ATCC 56-X.2). The cells do not grow well in semi-solid medium. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11 D13S317: 9 D16S539: 10,11 D5S818: 12 D7S820: 8 THO1: 8,9 TPOX: 9,11 vWA: 17 |
| Name of Depositor： | JG Rheinwald |
| Deposited As： | Homo sapiens |
| References： | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 |