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微信小章| 产品名称： | SCC-PSA1 |
|---|---|
| 商品货号： | TS210947 |
| Organism： | Mus musculus, mouse |
| Tissue： | testes |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pluripotent teratocarcinoma |
| Gender： | male |
| Strain： | 129/Sv |
| Applications： | The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503). In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates. Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor. |
| Clinical Data： | male |
| Comments： | NOTE - NO LIVE CULTURES CAN BE SENT. The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503). In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates. Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days. Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin. A somewhat variable percentage will attach. Change the medium every 2 to 3 days. Migration of the endodermal layer should begin within 24 to 36 hours after plating. Do not dislodge cells when changing the medium. The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. NOTE: Set up in advance flasks with irradiated fibroblast feeder layers (e.g., X-irradiated STO cells, ATCC 56-X™)
xa0 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |