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微信小章| 产品名称： | Sarcoma 180 |
|---|---|
| 商品货号： | TS210952 |
| Organism： | Mus musculus, mouse |
| Tissue： | sarcoma; derived from metastatic site: ascites |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Strain： | Swiss Webster |
| Tumorigenic： | Yes |
| Effects： | Yes |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105xa0cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Medium Renewal:xa0Every 2 to 3 days |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Dunham LJ, Stewart HL. A survey of transplantable and transmissible animal tumors. J. Natl. Cancer Inst. 13: 1299-1377, 1953. PubMed: 13035452 |