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微信小章| 产品名称： | SBAC |
|---|---|
| 商品货号： | TS210957 |
| Organism： | Bos taurus, cow |
| Tissue： | adrenal gland, cortex; zona fasciculata; zona reticularis |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Applications： | The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Receptor Expression： | adrenocorticotropic hormone (ACTH) |
| Comments： | The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division. The cells have a finite life span in culture (60 to 70 population doublings). Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994). |
| Complete Growth Medium： | Hams F12 medium with FGF (50 ng/ml of crude FGF - OR - 500 pg/ml of pure FGF), 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | MH Simonian |
| Deposited As： | Bos taurus |
| References： | Hornsby PJ, Gill GN. Hormonal control of adrenocortical cell proliferation. Desensitization to ACTH and interaction between ACTH and fibroblast growth factor in bovine adrenocortical cell cultures. J. Clin. Invest. 60: 342-352, 1977. PubMed: 194925 Simonian MH, et al. Clonal growth and culture life span of bovine adrenocortical cells in a serum-free medium. In Vitro Cell. Dev. Biol. 23: 247-252, 1987. PubMed: 3032889 Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Simonian MH, et al. Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium. Endocrinology 111: 919-927, 1982. PubMed: 6213403 Gospodarowicz D, et al. Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor. Endocrinology 100: 1080-1089, 1977. PubMed: 189990 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |