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| 产品名称： | S194/5.XXO.BU.1 |
|---|---|
| 商品货号： | TS210968 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | Resistant to 0.1 mM 5-bromo-2-deoxyuridine. Derived from the S194/5.XXO-1 cell line (ATCC TIB-19). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Derived from the S194/5.XXO-1 cell line (ATCC TIB-19). |
| Antigen Expression： | H-2d; PC.1 |
| Genes Expressed： | immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; not secreted |
| Comments： | Resistant to 0.1 mM 5-bromo-2-deoxyuridine. Derived from the S194/5.XXO-1 cell line (ATCC TIB-19). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgA (not secreted); kappa light chain (not secreted) |
| Name of Depositor： | R Hyman, P Ralph, S Sarkar |
| Deposited As： | Mus musculus |
| References： | Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 |