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| 产品名称： | RTH-149 |
|---|---|
| 商品货号： | TS210969 |
| Organism： | Oncorhynchus mykiss, trout, rainbow |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Comments： | Culture the cells at 21C. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 21.0°C |
| Name of Depositor： | JL Fryer |
| Deposited As： | Salmo gairdneri |
| References： | Lannan CN, et al. Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671-676, 1984. PubMed: 6542066 Fryer J, et al. A cell line derived from rainbow trout (Salmo gairdneri) hepatoma. Fish Pathol 15:193-200, 1981. |