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| 产品名称： | S3D3 3D3b |
|---|---|
| 商品货号： | TS210970 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites |
| Comments： | Animals were immunized with E. tenella sporozoites. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | American Cyanamid Co. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987 |