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| 产品名称： | S1A(Thy-1-b) |
|---|---|
| 商品货号： | TS210971 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Applications： | This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are resistant to 0.1 mM 5-bromo-2-deoxyuridine. |
| Derivation： | This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). |
| Genes Expressed： | The cells secrete a mutant Thy-1 glycoprotein. |
| Cellular Products： | The cells secrete a mutant Thy-1 glycoprotein. |
| Comments： | This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor. The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are resistant to 0.1 mM 5-bromo-2-deoxyuridine. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; heat-inactivated horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421 |