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| 产品名称： | S1A.TB.4.8.2 (replaces S1A.T4.G8) |
|---|---|
| 商品货号： | TS210974 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Applications： | This line was derived from the S1A lymphoma. The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2-deoxyuridine. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This line was derived from the S1A lymphoma. |
| Antigen Expression： | Thy-1.2 |
| Genes Expressed： | Thy-1.2 |
| Comments： | The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2-deoxyuridine. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | K Horibata, AW Harris |
| Deposited As： | Mus musculus |
| References： | Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550 |