微信小陈
微信小章
产品简介
购买须知
| 产品名称： | RR1022 Schmidt-Ruppin Sarcoma |
|---|---|
| 商品货号： | TS210978 |
| Organism： | Rattus norvegicus, rat |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Strain： | Amsterdam |
| Tumorigenic： | Yes |
| Effects： | Yes, in chickens (Injection of these cells into 2 week old chickens produced rapidly growing tumors, the cells of which possessed chicken chromosomes. The tumors apparently result from low levels of residual Rous sarcoma virus.) Yes, in newborn rats (Injection of as few as 10 cells results in rapidly growing tumors.) |
| Virus Resistance： | poliovirus 1, 2, 3 |
| Complete Growth Medium： | Medium 199 in Hanks BSS, 80%; bovine calf serum, 20%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove medium and allow the culture to sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | WW Nichols |
| Deposited As： | Rattus sp. |
| References： | . . Hereditas 50: 53-80, 1963. Injection of these cells into 2 week old chickens produced rapidly growing tumors, the cells of which possessed chicken chromosomes. The tumors apparently result from low levels of residual Rous sarcoma virus. Injection of as few as 10 cells results in rapidly growing tumors. |