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微信小章| 产品名称： | RPMI 7666 Gerner 7666 |
|---|---|
| 商品货号： | TS210979 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 32 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | HLA A3, Aw29, B7, B12; Blood type B+ |
| Genes Expressed： | immunoglobulin; Epstein-Barr virus (EBV) |
| Cellular Products： | immunoglobulin; Epstein-Barr virus (EBV) |
| Comments： | Biological Safety CLASS II. EBNA positive. The cells produce Epstein-Barr virus. |
| Complete Growth Medium： | RPMI 1640 medium, 80%; fetal bovine serum, 20%
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105xa0viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; CO2, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 9,11 D5S818: 11,12 D7S820: 8,11 THO1: 7,9.3 TPOX: 8,11 vWA: 14,16 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |