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| 产品名称： | RPMI-7951 |
|---|---|
| 商品货号： | TS210982 |
| Organism： | Homo sapiens, human |
| Tissue： | skin; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 18 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number= 49; range = 47 to 66. This is a hyperdiploid human cell line with the modal chromosome number of 49, occurring in 24% of cells. Polyploid cells occurred at 22%, which is high. Seven marker chromosomes were common to most cells, including t(10p14q); t(15q17p), t(Xp8p), del(17) (p13.1) and three others. Five others occurred only in some cells. Normal X chromosomes were absent. N14, N17 and N22 were mostly single-copied and N2 had three copies. Fluorescence examination did not show the presence of any Y-like chromosome. |
| Clinical Data： | 18 years Caucasian female |
| Antigen Expression： | Antigen expression: Blood Type A; Rh+ |
| Genes Expressed： | Blood Type A; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms pigmented melanoma |
| Comments： | A contaminant identified as Mycoplasma fermentans was eliminated in 1975. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 9,9.3 TPOX: 8 vWA: 17,19 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | G Moore |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1971 |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 J. Natl. Cancer Inst. 59: 301-307, 1977. Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479 |