微信小陈
微信小章| 产品名称： | RTG-2 |
|---|---|
| 商品货号： | TS210985 |
| Organism： | Oncorhynchus mykiss, trout, rainbow |
| Tissue： | mixed; testis; ovary |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male and female mixed |
| Storage Conditions： | liquid nitrogen vapor phase |
| Virus Resistance： | poliovirus 1 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Once per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005. |
| Culture Conditions： | Temperature: 22°C; cells do not survive above 26°C but will grow at temperatures as low as 4°C. |
| Name of Depositor： | K Wolf |
| Deposited As： | Salmo gairdneri |
| References： | . . Science 135: 1065, 1962. Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103 Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741 |
| Cross References： | Nucleotide (GenBank) : U82129 Oncorhynchus mykiss Y1 RNA, partial sequence. |