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微信小章| 产品名称： | RTgill-W1 |
|---|---|
| 商品货号： | TS210987 |
| Organism： | Oncorhynchus mykiss, trout, rainbow |
| Tissue： | gill |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Strain： | Walbaum |
| Applications： | The cell line may be used for in vitro toxicology, fish virology and fish biochemistry. |
| Karyotype： | heteroploid |
| Derivation： | RTgill-W1 was established from a 15 month old primary culture of apparently normal rainbow trout gill fragments. |
| Virus Susceptibility： | Infectious salmon anemia virus
,
Infectious salmon anemia virus |
| Comments： | The cell line supports the replication of infectious salmon anemia (ISA) virus but CPE is not observed. RTgill-W1 is a modest generator of eicosanoids in that only low levels of thromboxane B2 and prostaglandin E2 are detected while no lipoxygenase products are observed.
Prior to deposit at ATCC, the line was found to be contaminated with mycoplasma, and was cured by treatment with BM-Cycline and mycoplasma removal agent (MRA). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:4 Medium Renewal: Twice a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 19°C; (Max. 20°C, Min. 18°C)
Atmosphere: air, 100% |
| Name of Depositor： | NC Bols |
| Deposited As： | Oncorhynchus mykiss |
| References： | Bols NC, et al. Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills. J. Fish Dis. 17: 601-611, 1994. Falk K, et al. Characterization of infectious salmon anemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.). J. Virol. 71: 9016-9023, 1997. PubMed: 9371558 Schirmer K, et al. Ability of 16 priority PAHs to be photocytotoxic to a cell line from the rainbow trout gill. Toxicology 127: 143-155, 1998. PubMed: 9699801 Holland JW, et al. The eicosanoid generating capacity of isolated cell populations from the gills of the rainbow trout, Oncorhynchus mykiss. Comp. Biochem. Physiol. C. Pharmacol. Toxicol. Endocrinol. 122: 297-306, 1999. PubMed: 10336089 Schirmer K, et al. Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill. Toxicology 127: 129-141, 1998. PubMed: 9699800 |