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微信小章| 产品名称： | RPMI 6666 |
|---|---|
| 商品货号： | TS210994 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cell contain Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Hodgkins disease; Hodgkins lymphoma |
| Age： | 29 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | 29 years Caucasian male |
| Antigen Expression： | HLA A2, A3, B7, B18 |
| Genes Expressed： | Immunoglobulin |
| Cellular Products： | Immunoglobulin |
| Comments： | The cells are EBNA positive. |
| Complete Growth Medium： | RPMI 1640 medium, 80%; fetal bovine serum, 20%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO 5% Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Moore GE, et al. Leukocyte cultures of patients with leukemia and lymphomas. N.Y. State J. Med. 66: 2757-2764, 1966. PubMed: 5232150 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |