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微信小章| 产品名称： | RPC5.4 |
|---|---|
| 商品货号： | TS210995 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma. The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine. Tested and found negative for ectromelia virus (mousepox). |
| Karyotype： | modal number = 60 |
| Derivation： | The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma. |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma. The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | B Mohit |
| Deposited As： | Mus musculus |
| References： | Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030 Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Schwaber J. Human lymphocyte -- mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genet. 3: 295-302, 1977. PubMed: 305122 |