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微信小章| 产品名称： | RPMI 1788 |
|---|---|
| 商品货号： | TS210997 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 33 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | human male; hypodiploid; stable |
| Antigen Expression： | HLA A2, Aw33, B7, B14 |
| Genes Expressed： | immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta) |
| Cellular Products： | immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta) |
| Comments： | The cells are EBNA positive. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106xa0cells/mL. Do not allow the cell concentration to exceed 2 x 106xa0cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature:xa037°Cxa0 |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 10,12 THO1: 6,9.3 TPOX: 8,9 vWA: 18,19 |
| Isoenzymes： | G6PD, B |
| Isotype： | IgM; lambda light chain |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523 Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807 Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |