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微信小章| 产品名称： | RmcB |
|---|---|
| 商品货号： | TS210999 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells. Spleen cells were fused with Sp2/0 myeloma cells. The RmcB cell line was established in 1985 by R. L. Crowell. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor) |
| Comments： | A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
The antibody blocks binding and infection by coxsackie B Virus, but not adenovirus.
Mouse monoclonal antibody (RmcB) protects HeLa cells and Buffalo green monkey kidney cells from infection by all six serotypes of CB. RmcB did not protect HeLa cells from infection by poliovirus, echovirus 6, or coxsackievirus A18. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 9 x 105 cells/mL. Do not allow the cell concentration to exceed 9 x 105xa0 cells/mL. Medium Renewal:xa0xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | RW Finberg |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1985 |
| References： | Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860 Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580 Hsu KH, et al. A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |