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| 产品名称： | RLTM01 Anti-PAP 1, DB87 8G2.7.4 |
|---|---|
| 商品货号： | TS211001 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with purified PAP from human semen. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human prostatic acid phosphatase (PAP) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human prostatic acid phosphatase (PAP) |
| Comments： | Animals were immunized with purified PAP from human semen. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Name of Depositor： | Cetus Corp. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | MTimkulu T, et al. Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay. US Patent 4,690,890 dated Sep 1 1987 |