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微信小章| 产品名称： | RKO |
|---|---|
| 商品货号： | TS211011 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinoma |
| Applications： | It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters. |
| Derivation： | RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain. |
| Receptor Expression： | urokinase receptor (u-PAR) Ref   Boyd D, et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 2835152 |
| Oncogene： | p53 + (wild type)
RefSmith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Yes, in soft agar |
| Comments： | RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (TS211011) cells than in RKO-E6 (ATCC CRL-2578) cells. The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:12 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
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| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X
CSF1PO: 8, 10, 11
D13S317: 8, 11
D16S539: 12, 13
D5S818: 11, 13, 15
D7S820: 8, 10
THO1: 6, 10
TPOX: 11
vWA: 15, 16, 17, 22 |
| Name of Depositor： | MC Hollander, AJ Fornace |
| Deposited As： | human |
| References： | Boyd D, et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 2835152 Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 Brattain MG, et al. Heterogeneity of human colon carcinoma. Cancer Metastasis Rev. 3: 177-191, 1984. PubMed: 6437669 Bhat MK, et al. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed: 9360971 Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 |