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微信小章| 产品名称： | RL-65 |
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| 商品货号： | TS211016 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | lung |
| Cell Type： | Squamous Epithelium,Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 5 days |
| Strain： | Sprague-Dawley |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed： | cytoskeletal proteins (alpha keratin, actin, desmin, vimentin, tubulin); fibronectin; laminin |
| Cellular Products： | cytoskeletal proteins (alpha keratin, actin, desmin, vimentin, tubulin); fibronectin; laminin |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium. |
| Comments： | The cells are maintained in a serum free medium. If grown in media containing serum, the properties of the cells will change. The cells exhibit different characteristic when grown with or without retinoic acid. In the presence of retinoic acid (50 nM), the cells resemble low non-keratinized or squamous epithelium with densely packed colonies. In the absence of retinoic acid, the cells form a keratinized epithelium. Long term cultures in the absence of retinoic acid form a stratified highly keratinized epithelium with large networks of epithelial filaments. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 1.2 g/L sodium bicarbonate and supplemented with 0.005 mg/ml insulin, 0.01 mg/ml human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, and bovine pituitary extract (0.15 mg protein per ml).
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Genentech, Inc. |
| Deposited As： | Rattus sp. |
| U.S. Patent Number： | |
| References： | Mather JP, Roberts PE. Method of isolating lung cell line. US Patent 5,364,785 dated Nov 15 1994 Roberts PE, et al. A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype. Am. J. Physiol. 259: L415-L425, 1990. PubMed: 2260675 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |