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微信小章| 产品名称： | RL95-2 |
|---|---|
| 商品货号： | TS211017 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus; endometrium |
| Product Format： | frozen |
| Morphology： | epithelial-like and/or rounded |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 65 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | 65 years Caucasian female |
| Receptor Expression： | estrogen receptor, positive |
| Comments： | The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 8,12 D16S539: 11,13 D5S818: 10,11 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 16,20 |
| Name of Depositor： | DL Way |
| Deposited As： | Homo sapiens |
| References： | Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371 Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448 |