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| 产品名称： | RF/6A |
|---|---|
| 商品货号： | TS211022 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | choroid; retina |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Applications： | Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
|
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line spontaneously transformed at an early age and has been passaged over 540 times. |
| Genes Expressed： | factor VIII; fibronectin |
| Comments： | Weibel-Palade granules are seen by electron microscopy. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37°C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | F Hu |
| Deposited As： | Macaca mulatta |
| References： | Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454 |