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微信小章| 产品名称： | RIN-14B |
|---|---|
| 商品货号： | TS211026 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | pancreas, islet cell tumor |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male |
| Strain： | NEDH |
| Applications： | These cellsxa0offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The RIN-14B cell line is a secondary clone derived from the RIN-m rat islet line (ATCC CRL-2057 ). |
| Genes Expressed： | Somatostatin; L-dopa-decarboxylase |
| Cellular Products： | Somatostatin; L-dopa-decarboxylase |
| Comments： | The cells produce and secrete somatostatin, and produce L-dopa-decarboxylase (a marker for cells having amine precursor uptake and decarboxylation, or APUD, activity). Unlike the parental line they do not produce insulin. The parental line was also cloned to establish RIN-5F (ATCC CRL-2058) that produces only insulin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | AF Gazdar, H Oie |
| Deposited As： | Rattus sp. |
| References： | Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566 Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819 Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859 Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192 Oie HK, et al. Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. |