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| 产品名称： | RC-4B/C |
|---|---|
| 商品货号： | TS211033 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | pituitary, anterior |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenoma |
| Age： | 3 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was carried continuously in culture for 5 years before it was sucesssfully cryopreserved. |
| Clinical Data： | male |
| Receptor Expression： | gonadotropin releasing hormone (GnRH) |
| Genes Expressed： | luteinizing hormone beta (LH); growth hormone (GH); follicle stimulating hormone beta (FSH); prolactin; adrenal corticotropic hormone (ACTH); thyrotropin beta |
| Cellular Products： | luteinizing hormone beta (LH); growth hormone (GH); follicle stimulating hormone beta (FSH); prolactin; adrenal corticotropic hormone (ACTH); thyrotropin beta |
| Comments： | The line contains cells producing LH, GH, FSH, prolactin, ACTH and thyrotropin beta (hormone production was tested using cells that were grown in medium containing Nu-serum rather than fetal bovine serum).
GnRH receptors are about 2 fold lower in number than on normal pituitary.
The cells contain numerous electron dense secretory granules, golgi complexes, and extended arrays of rough endoplasmic reticulum as well as endogenous rat C-type retrovirus particles.
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 45%; alpha minimum essential medium with 1 g/L glucose, 45%; supplemented with 0.01 mM nonessential amino acids, 15 mM HEPES, 0.2 mg/ml bovine serum albumin, 2.5 ng/ml epidermal growth factor; dialyzed heat-inactivated fetal bovine serum, 10%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | EH Leiter |
| Deposited As： | Rattus sp. |
| References： | Hurbain-Kosmath I, et al. Gonadotropes in a novel rat pituitary tumor cell line, RC-4B/C. Establishment and partial characterization of the cell line. In Vitro Cell. Dev. Biol. 26: 431-440, 1990. PubMed: 2161825 |