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| 产品名称： | RD-ES |
|---|---|
| 商品货号： | TS211036 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | mixed: adherent and clusters in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Ewings sarcoma |
| Age： | 19 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Derivation： | The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus. |
| Clinical Data： | 19 years Caucasian male |
| Antigen Expression： | Blood type B; Rh+ |
| Comments： | Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter. The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing： | Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | G Marshall, M Kirchen |
| Deposited As： | Homo sapiens |