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微信小章| 产品名称： | Ramos (RA 1) |
|---|---|
| 商品货号： | TS211039 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma (American) |
| Age： | 3 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Clinical Data： | 3 years Caucasian male |
| Antigen Expression： | CD23 + Ref   Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitts lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726 |
| Receptor Expression： | interleukin 4 (IL-4); low affinity IgE (CD23) Ref Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitts lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726 |
| Genes Expressed： | immunoglobulin (surface and secreted)
Ref
Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763xa0RefKlein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343 |
| Cellular Products： | immunoglobulin (surface and secreted)
Ref Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763RefKlein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343 |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells are negative for Epstein-Barr virus.
Both the parental line (TS211039) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.
The cells are reported to secrete IgM (lambda light chain). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL.
Maintain cell density between 2 X 105 and 1 X 106 viable cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 10,13 D5S818: 7,12 D7S820: 11 THO1: 7,9.3 TPOX: 8,9 vWA: 15,16 |
| Isotype： | IgM; lambda light chain |
| Name of Depositor： | BC Giovanella |
| Deposited As： | Homo sapiens |
| References： | Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343 Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879 Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitts lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726 Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896 Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 |