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微信小章| 产品名称： | Raji |
|---|---|
| 商品货号： | TS211041 |
| Organism： | Homo sapiens, human |
| Tissue： | lymphoblast |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Herpesvirus (EBV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 11 years |
| Gender： | male |
| Ethnicity： | Black |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra "E" group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome. |
| Derivation： | The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitts lymphoma of the left maxilla of an 11-year-old Black male. |
| Clinical Data： | 11 years Black male The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitts lymphoma of the left maxilla of an 11-year-old Black male. |
| Comments： | These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4xa0x 105 viable cells/mL. A maximum of 3 X 106 viable cells/mL is obtainable.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 8,11 D5S818: 10,13 D7S820: 10 THO1: 6,7 TPOX: 8,13 vWA: 16,19 |
| Name of Depositor： | W Henle |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1963 |
| References： | Pulvertaft JV. Cytology of Burkitts tumour (African lymphoma). Lancet 1: 238-240, 1964. PubMed: 14086209 Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitts lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposis sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568 Epstein MA, et al. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547-559, 1966. PubMed: 4288580 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Rich SA, et al. Purification, microresequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions. J. Biol. Chem. 271: 1118-1126, 1996. PubMed: 8557639 U.S. Pharmacopeia USP Monographs: Technetium 99mTc Fanolesomab Injection. Rockville, MD: USP32-NF27, 2005 |