微信小陈
微信小章
产品简介
购买须知
| 产品名称： | RAN-2 alpha RAN-2, anti RAN-2 |
|---|---|
| 商品货号： | TS211044 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2) |
| Comments： | Animals were immunized with enriched preparations of rat astrocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody does not react with cultured neurons, oligodendrocytes, Schwann cells, microglia or various non-neuronal cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | PF Bartlett, M Raff |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Bartlett PF, et al. Rat neural antigen-2 (RAN-2): a cell surface antigen on astrocytes, ependymal cells, Muller cells and lepto-meninges defined by a monoclonal antibody. Brain Res. 204: 339-351, 1980. PubMed: 7006734 |