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微信小章| 产品名称： | Raszip 6 |
|---|---|
| 商品货号： | TS211045 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | This line was established by G.P. Dotto, et al. The cells produce a high titer of helper free retrovirus carrying the vHa-ras oncogene and the G418 resistance gene. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was established by G.P. Dotto, et al. in 1985 by transfecting psi2 cells with the raszip 6 DNA construct. |
| Comments： | This line was established by G.P. Dotto, et al. in 1985 by transfecting psi2 cells with the raszip 6 DNA construct. The cells produce a high titer of helper free retrovirus carrying the vHa-ras oncogene and the G418 resistance gene. |
| Complete Growth Medium： | Dulbeccos modified Eagles Medium with 4.5 g/L glucose and 0.5 mg/ml G418, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 5th edition, published byxa0Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RA Weinberg |
| Deposited As： | Mus musculus |
| Year of Origin： | 1985 |
| References： | Dotto GP, et al. Specific growth response of ras-transformed embryo fibroblasts to tumour promoters. Nature 318: 472-475, 1985. PubMed: 4069218 Mann R, et al. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33: 153-159, 1983. PubMed: 6678608 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |