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| 产品名称： | R6.5.D6.E9.B2 R6-5-D6 |
|---|---|
| 商品货号： | TS211048 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Strain： | BALB/c |
| Applications： | The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1) |
| Comments： | Animals were immunized with the EBV transformed lymphoblast cell line, JY. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml. Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | mouse IgG2a kappa |
| Population Doubling Time： | 12 hours |
| Name of Depositor： | Dana Farber Cancer Inst. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994 Mice were immunized with the EBV-transformed human lymphoblastoid cell line JY. The antibody binds to ICAM-1(CD54), and inhibits the interaction of ICAM-1 with leukocytes via lymphocyte function antigen 1(LFA-1, CD11a/CD18). |