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| 产品名称： | R2-9A5 R2-9A5.2A2.1A10 |
|---|---|
| 商品货号： | TS211051 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The hybridoma cell line R2-9A5 R2-9A5.2A2.1A10 was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis. |
| Derivation： | The hybridoma cell line R2-9A5 R2-9A5.2A2.1A10 was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. |
| Clinical Data： | R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75 |
| Comments： | The hybridoma cell line R2-9A5 R2-9A5.2A2.1A10 was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis. R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75. |
| Complete Growth Medium： | Iscoves Modified Dulbeccos Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2b |
| Name of Depositor： | DH Presky |
| Deposited As： | rat |
| Year of Origin： | 1993 |
| References： | Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576 |