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微信小章| 产品名称： | R2C |
|---|---|
| 商品货号： | TS211053 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | testis |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leydig cell tumor |
| Age： | 2 months |
| Gender： | male |
| Strain： | WFu |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Genes Expressed： | steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone |
| Cellular Products： | steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone |
| Virus Resistance： | poliovirus 2 |
| Comments： | Stimulation by other hormones or cAMP is not required for hormone secretion. Reverse Transcript positive |
| Complete Growth Medium： | The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | G Sato |
| Deposited As： | Rattus sp. |
| References： | Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |