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微信小章| 产品名称： | R4-6A2 |
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| 商品货号： | TS211054 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages.xa0 The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon. 30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with partially purified murine gamma interferon.xa0 Spleen cells were fused with P3U-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse gamma interferon |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse gamma interferon |
| Tumorigenic： | Yes |
| Effects： | Forms ascites in nude mice. |
| Comments： | The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages.xa0 The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon. 30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104xa0viable cells/mL. Maintain cultures at a cell concentration between 6 x 104xa0and 6 x 105xa0cells/mL. Do not allow the cell concentration to reach 1 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days
Note: Do not allow the cell concentration to reach 1 x 106 cells/mL. |
| Cryopreservation： | Complete culture medium, 92.5%; DMSO, 7.5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | GL Spitalny |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560-1565, 1984. PubMed: 6425450 Tay CH, Welsh RM. Distinct organ-dependent mechanisms for the control of murine cytomegalovirus infection by natural killer cells. J. Virol. 71: 267-275, 1997. PubMed: 8985346 Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913 Skeen MJ, et al. Regulation of murine macrophage IL-12 production. J. Immunol. 156: 1196-1206, 1996. PubMed: 8557998 Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |