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| 产品名称： | R1.1 |
|---|---|
| 商品货号： | TS211060 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C58/J |
| Applications： | Established from a spontaneous thymoma. Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Established from a spontaneous thymoma. |
| Antigen Expression： | H-2k; Thy-1.2; TL |
| Genes Expressed： | H-2k; Thy-1.2; TL |
| Comments： | Established from a spontaneous thymoma. Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | P Ralph |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 |