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微信小章| 产品名称： | R1-2 |
|---|---|
| 商品货号： | TS211062 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with the Peyers patch HEV binding lymphoma line TK1. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyers patch HEV adhesion molecule), VLA-4 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyers patch HEV adhesion molecule), VLA-4 |
| Comments： | The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyers patch. This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical). |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Isotype： | IgG2b |
| Name of Depositor： | IL Weissman |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Holzmann B, et al. Identification of a murine Peyers patch-specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4alpha. Cell 56: 37-46, 1989. PubMed: 2463092 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |