微信小陈
微信小章
产品简介
购买须知
| 产品名称： | R17 208.2 |
|---|---|
| 商品货号： | TS211064 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Erythroleukemia |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse transferrin receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse transferrin receptor |
| Comments： | Animals were immunized with the mouse erythroleukemia line 745.6. Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. This antibody blocks iron uptake and cell proliferation of most murine hematopoietic cell lines and primary cell cultures. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum |
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Two to four times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgM , IgM |
| Name of Depositor： | J Lesley |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505 |