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产品简介
购买须知
| 产品名称： | R1E/TL8x.1 |
|---|---|
| 商品货号： | TS211067 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C58/J |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2k; Thy-1.2 |
| Genes Expressed： | H-2k; Thy-1.2 |
| Comments： | Unlike the parental line R1.1, these cells do not express TL antigen. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: culture medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. derived from R1.1 (ATCC TIB-42). |