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微信小章| 产品名称： | QNR/D |
|---|---|
| 商品货号： | TS211070 |
| Organism： | Coturnix coturnix japonica, quail, Japanese |
| Tissue： | neuroretina |
| Cell Type： | neuronalinfected with Rous sarcoma virus mutant ts NY-68 |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | embryo, 7 days gestation |
| Applications： | Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish the QNR ts NY-68 mixed cell line. QNR ts NY-68 was subsequently cloned to establish the QNR/D cell line. The cells are routinely maintained at 38.5 to 39C. The permissive temperature for transformation is 36C. Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. The cell line displays properties of amacrine and/or ganglion cells. QNR/D cells can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina. QNR/D cells (TS211070) and QNR/K2 cell (ATCC CRL-2533) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 38°C to 39°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | B Pessac, D Trisler |
| Deposited As： | Coturnix coturnix japonica |
| References： | Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691 Trisler D, et al. Retinal engineering: engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804 Cohen-Salmon M, et al. Characterization of the promoter of the human KAL gene, responsible for the X-chromosome-linked Kallmann syndrome. Gene 164: 235-242, 1995. PubMed: 7590336 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |