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微信小章| 产品名称： | QT6 |
|---|---|
| 商品货号： | TS211072 |
| Organism： | Coturnix coturnix japonica, quail, Japanese |
| Cell Type： | methylcholanthrene induced |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrosarcoma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Derived from chemically induced tumors of Japanese quail. |
| Tumorigenic： | Yes, in animal host. |
| Effects： | Yes, in animal host and soft agar. |
| Virus Susceptibility： | Rous sarcoma virus
Avian encephalomyelitis virus Rabies virus Mareks disease virus |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
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| Subculturing： | Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in a trypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive. Note: When subcultures are prepared using pipetting or mechanical means the cells adhere lightly to the flask substrate and exhibit a tendency toward clumping.xa0 Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | C Moscovici |
| Deposited As： | Coturnix coturnix japonica |
| References： | Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709 Imagawa S, et al. Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89: 1430-1439, 1997. PubMed: 9028967 Martin PT, Sanes JR. Integrins mediate adhesion to agrin and modulate agrin signaling. Development 124: 3909-3917, 1997. PubMed: 9367446 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |