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| 产品名称： | PtK2 (NBL-5) |
|---|---|
| 商品货号： | TS211078 |
| Organism： | Potorous tridactylus, potoroo |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | adult |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Virus Susceptibility： | Human Coxsackievirus A 9
Herpes simplex virus Vaccinia virus Vesicular stomatitis New Jersey virus |
| Virus Resistance： | adenovirus 5; coxsackievirus B5; poliovirus 2 |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | KH Whalen |
| Deposited As： | Potorous tridactylis |
| References： | Walen KH. Spatial Relationships in the Replication of Chromosomal DNA. Genetics 51: 915-929, 1965. PubMed: 14337764 |